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doxycycline inducible cas9 expression cassette  (Addgene inc)


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    Structured Review

    Addgene inc doxycycline inducible cas9 expression cassette
    Doxycycline Inducible Cas9 Expression Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 349 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/doxycycline+inducible+cas9+expression+cassette/pCW-Cas9+(Plasmid+%2350661)/pmc12184417__pnas%2E2424409122%2Esapp-213-7-17
    Average 96 stars, based on 349 article reviews
    doxycycline inducible cas9 expression cassette - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium
    Article Snippet: .. One donor plasmid (Puro-Cas9, Addgene plasmid no. 58409) comprised a doxycycline-inducible Cas9 expression cassette selectable with puromycin, the other (Neo-M2rtTA, Addgene plasmid no. 60843) contained a constitutive reverse tetracycline transactivator selectable with G418 (Geneticin). ..

    Article Title: Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction
    Article Snippet: .. RA3087 FANCA −/− human fibroblasts and Fan1 −/− MEFs were transduced with lentivirus expressing pCW-Cas9 vector encoding a doxycycline-inducible Cas9 expression cassette (plasmid 50661; Addgene; originally from the Lander and Sabatini laboratories, Massachusetts Institute of Technology). ..

    Expressing:

    Article Title: CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium
    Article Snippet: .. One donor plasmid (Puro-Cas9, Addgene plasmid no. 58409) comprised a doxycycline-inducible Cas9 expression cassette selectable with puromycin, the other (Neo-M2rtTA, Addgene plasmid no. 60843) contained a constitutive reverse tetracycline transactivator selectable with G418 (Geneticin). ..

    Article Title: CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) (12). ..

    Article Title: The Role of ATP Synthase Subunit e (ATP5I) in Mediating the Metabolic and Antiproliferative Effects of Biguanides
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Article Title: Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction
    Article Snippet: .. RA3087 FANCA −/− human fibroblasts and Fan1 −/− MEFs were transduced with lentivirus expressing pCW-Cas9 vector encoding a doxycycline-inducible Cas9 expression cassette (plasmid 50661; Addgene; originally from the Lander and Sabatini laboratories, Massachusetts Institute of Technology). ..

    Generated:

    Article Title: CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) (12). ..

    Article Title: The Role of ATP Synthase Subunit e (ATP5I) in Mediating the Metabolic and Antiproliferative Effects of Biguanides
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Transduction:

    Article Title: CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) (12). ..

    Article Title: The Role of ATP Synthase Subunit e (ATP5I) in Mediating the Metabolic and Antiproliferative Effects of Biguanides
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Article Title: Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction
    Article Snippet: .. RA3087 FANCA −/− human fibroblasts and Fan1 −/− MEFs were transduced with lentivirus expressing pCW-Cas9 vector encoding a doxycycline-inducible Cas9 expression cassette (plasmid 50661; Addgene; originally from the Lander and Sabatini laboratories, Massachusetts Institute of Technology). ..

    Genome Wide:

    Article Title: CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) (12). ..

    Article Title: The Role of ATP Synthase Subunit e (ATP5I) in Mediating the Metabolic and Antiproliferative Effects of Biguanides
    Article Snippet: .. Briefly, a NALM-6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Gene Knockout:

    Article Title: CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated doxycycline-inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs) (12). ..



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    96
    Addgene inc doxycycline inducible cas9 expression cassette
    Doxycycline Inducible Cas9 Expression Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/doxycycline+inducible+cas9+expression+cassette/pCW-Cas9+(Plasmid+%2350661)/pmc12184417__pnas%2E2424409122%2Esapp-213-7-17
    Average 96 stars, based on 1 article reviews
    doxycycline inducible cas9 expression cassette - by Bioz Stars, 2026-11
    96/100 stars
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